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The Association of miR-let 7b and miR-548 with PTEN in Prostate Cancer

Mojtaba Saffari, Sayyed Mohammad Hossein Ghaderian, Mir Davood Omrani, Mandana Afsharpad, Kimia Shankaie, Niusha Samadaian
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Abstract

Purpose: This study aims to investigate the expression level of mir-let7b-3p and mir-548, which are involved in PTEN expression in tissue samples of prostate cancer patients versus benign prostate hyperplasia (BPH) and normal adjacent tissue.

Materials and Methods: Prostate cancer tissues were obtained from patients after receiving informed consent. Total RNA extraction and cDNA synthesis were performed for determining gene expression.

Results: Ten patients were determined to have high Gleason scores (> 7), 36 and seven samples had intermediate Gleason scores (7?) and BPH, respectively, and 40 samples were derived from normal adjacent tissue. Downreg­ulation of mir-let7b and upregulation of mir-548 expression significantly correlated with high-risk Gleason scores.

Conclusion: The present study showed that miR-let7b and/or mir-548 can be considered as potential targets in prostate cancer therapy.


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DOI: http://dx.doi.org/10.22037/uj.v0i0.4564

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