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Assessment of Sperm PAWP Expression in Infertile Men

Somayeh Tanhaei, Solmaz Abdali-Mashhadi, Marziyeh Tavalaee, Soudabeh Javadian-Elyaderani, Kamran Ghaedi, Seyed Morteza Seifati, Mohammad H. Nasr-Esfahani



Purpose: The aim of this study was to evaluate expression of Post-Acrosomal WW Binding Protein (PAWP) in infertile men with low and high fertilization post ICSI and also globozoospermic men.

Materials and Methods: Semen samples were collected from 18 infertile men with previously failed or low fertili­zation (< 25%) post ICSI, 10 men with high fertilization (>50%) post ICSI, 15 globozoospermic men, and 21 fertile individuals. Then, expression of PAWP was assessed at RNA with quantitative Real Time PCR.

Results: Relative expression of PAWP in sperm was significantly (P < .05) lower in infertile men with globozo­ospermia (41.5 ± 5.7) or low fertilization rate (43.3±10.4) compared to fertile (138.8 ± 17.3)or men with high fer­tilization (211.6 ± 75.6). In addition, a significant positive correlation (r = 0.628; P = .001) was observed between percentage of fertilization with the relative expression of PAWP.

Conclusion: Considering solid recent evidences regarding PLC? as the main sperm factor involved in oocyte acti­vation, therefore co-localization of PLC? with PAWP in perinuclear theca may account for the above observation and it is likely that PAWP may have other functions and/or it may assist PLC?.



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DOI: http://dx.doi.org/10.22037/uj.v0i0.4621


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