Purpose: The aim of this study was to evaluate expression of Post-Acrosomal WW Binding Protein (PAWP) in infertile men with low and high fertilization post ICSI and also globozoospermic men.
Materials and Methods: Semen samples were collected from 18 infertile men with previous failed or low fertilization (<25%) post ICSI, 10 men with high fertilization (>50%) post ICSI, 15 globozoospermic men, and 21 fertile individuals. Then, expression of PAWP was assessed at RNA with quantitative Real Time PCR.
Results: Relative expression of PAWP in sperm was significantly (p<0.05) lower in infertile men with globozoospermia (41.5±5.7) or low fertilization rate (43.3±10.4) compared to fertile (138.8±17.3)or men with high fertilization (211.6±75.6). In addition, a significant positive correlation (r=0.628; p=0.001) was observed between percentage of fertilization with the relative expression of PAWP.
Conclusion: Considering solid recent evidences regarding PLC? as the main sperm factor involved in oocyte activation, therefore co-localization of PLC? with PAWP in perinuclear theca may account for the above observation and it is likely that PAWP may have other functions and/or it may assist PLC?.
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