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Comparison of the Histopathological Findings of Testis Tissues of Non-Obstructive Azoospermia with the Findings after Microscopic Testicular Sperm Extraction

Serdar Toksöz, Yalçin Kizilkan
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Abstract

 Purpose: To investigate the relationship between the histopathological findings of testis tissue samples and sperm retrieval success of micro-TESE in non-obstructive azoospermia (NOA) patients.

Method: Histopathological examination results of the testis tissue samples of 795 NOA patients who underwent micro-TESE operation in our clinic between 2003 and 2014 were included. Histopathological findings were grouped as hypospermatogenesis, incomplete spermatocytic arrest, complete spermatocytic arrest, Sertoli cell only syndrome (SCOS), and fibrosis/atrophy. Chi-square analysis was used to compare the histopathological findings with the sperm retrieval rates of micro-TESE.

Result: Sperm was found in 341 (42,9%) patients following micro-TESE compared to 454(57,1%) patients where sperm were not detected (P < 0.001). Sperm retrieval rates of micro TESE were significantly higher in hyposper­matogenesis and incomplete maturation arrest groups (93.2% (P < 0.001) and 72.5% (P < 0.001), respectively). Complete maturation arrest, SCOS and fibrosis/atrophy were determined at significantly higher rates in patients (220.2%) with no sperm found compared to patients with sperm (P < 0.001).

Conclusion: The findings of this study are consistent with those of previous studies in the literature. Testicular histopathological findings can provide additional data when informing NOA patients about the expected success of further micro-TESE operations.


References

World Health Organization. WHO Manual for the Standardized Investigation and Diagnosis of the Infertile Couple. Cambridge: Cambridge University Press, 2000. http://www.who.int/reproductivehealth/publications/infertility/0521774748/en/

Willott GM. Frequency of azoospermia. Forensic Sci Int 1982;20: 9-10.

Jarow JP, Espeland MA, Lipshultz LI. Evaluation of the azoospermic patient. J Urol 1989;142:62-5.

Palermo G, Joris H, Devroey P, Van Steirteghem AC. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet 1992 ; 340: 17-8.

Hopps CV, Goldstein M, Schlegel PN. The diagnosis and treatment of the azoospermic patient in the age of intracytoplasmic sperm injection. Urol Clin North Am 2002; 29: 895-911.

Sabanegh E AA. Male Infertility. In: Wein AJ KL, Novick AC, Partin AW, Peters CA, editor. Campbell-Walsh Urology. 10th ed. Philadelphia: Saunders, Elsevier Inc.; 2012. p. 616-47.

Jungwirth A1, Giwercman A, Tournaye H, Diemer T, et all; European Association of Urology guidelines on Male Infertility. Eur Urol. 2012 Aug;62(2):324-32.

Dabaja AA, Schlegel PN. Microdissection testicular sperm extraction: an update. Asian J Androl 2013; 15: 35-9.

Schlegel PN, Su LM Physiological consequences of testicular sperm extraction. Hum Reprod. 1997 Aug;12(8):1688-92.

Salehi P, Derakhshan-Horeh M, Nadeali Z, Hosseinzadeh M, Sadeghi E, Izadpanahi MH, Salehi M. Factors influencing sperm retrieval following testicular sperm extraction in nonobstructive azoospermia patients. Clin Exp Reprod Med. 2017 Mar;44(1):22-27

Kuai YR, He ZJ, Wang S, Zhang K, Zeng C, Chen L, Xue Q, Shang J, Yang HX, Xu Y. Testicular histology does not affect the clinical outcomes of ICSI in men with non-obstructive azoospermia. Zhonghua Nan Ke Xue. 2017 Oct;23(10):889-893.

Gul U, Turunc T, Haydardedeoglu B, Yaycioglu O, Kuzgunbay B, Ozkardes H. Sperm retrieval and live birth rates in presumed Sertoli-cell-only syndrome in testis biopsy: a single centre experience. Andrology. 2013 Jan;1(1):47-51.

Eken A, Gulec F. Microdissection testicular sperm extraction (micro-TESE): Predictive value of preoperative hormonal levels and pathology in non-obstructive azoospermia. Kaohsiung J Med Sci. 2018 Feb;34(2):103-108.

Guler I, Erdem M, Erdem A, Demirdağ E, Tunc L, Bozkurt N, Mutlu MF, Oktem M. Impact of testicular histopathology as a predictor of sperm retrieval and pregnancy outcome in patients with nonobstructive azoospermia: correlation with clinical and hormonal factors. Andrologia. 2016 Sep;48(7):765-73.




DOI: http://dx.doi.org/10.22037/uj.v16i2.4756

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